The Function of GLI3
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Protein names
Recommended name:
Transcriptional activator GLI3Short name:
GLI3FLAlternative name(s):
GLI3 form of 190 kDaGLI3-190
GLI3 full length protein
GLI3 C-terminally truncated form
GLI3 form of 83 kDa
GLI3-83
- RS10278194 (GLI3) ??
- RS12532960 (GLI3) ??
- RS2049622 (GLI3) ??
- RS4724100 (GLI3) ??
- RS7710527 (GLI3) ??
- RS846271 (GLI3) ??
- RS929387 (GLI3) ??
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Top Gene-Substance Interactions
GLI3 Interacts with These Diseases
Disease | Score |
Substances That Increase GLI3
Substances | Interaction | Organism | Category |
Substances That Decrease GLI3
Substances | Interaction | Organism | Category |
Advanced Summary
Greig cephalopolysyndactyly syndrome Several types of mutations in the GLI3 gene have been identified in people with Greig cephalopolysyndactyly syndrome. These genetic changes include insertions or deletions of a small amount of DNA and changes in single DNA building blocks (base pairs) in critical regions of the gene. In other cases, this condition is caused by chromosomal abnormalities involving the region of chromosome 7 that contains the GLI3 gene. The genetic changes that cause Greig cephalopolysyndactyly syndrome prevent one copy of the gene in each cell from producing any functional GLI3 protein. As a result, only half the normal amount of this protein is available to control the expression of target genes during embryonic development. It remains unclear how a reduced amount of the GLI3 protein disrupts development of the limbs, head, and face and causes the specific features of Greig cephalopolysyndactyly syndrome. Pallister-Hall syndrome Most of the mutations responsible for Pallister-Hall syndrome occur near the middle of the GLI3 gene. These genetic changes typically create a premature stop signal in the instructions for making the GLI3 protein. As a result, cells produce an unusually short version of the protein. Unlike the full-length GLI3 protein, which can turn target genes on or off, the short protein can only turn off (repress) the expression of target genes. Although this defect clearly disrupts aspects of embryonic development, it is not known how the altered function of the GLI3 protein leads to the varied signs and symptoms of Pallister-Hall syndrome. other disorders Mutations in the GLI3 gene have been found in people with several forms of polydactyly (the presence of extra fingers and/or toes). These cases are described as isolated or nonsyndromic because the polydactyly occurs without other signs and symptoms, such as brain abnormalities or widely spaced eyes. GLI3 mutations can cause two types of polydactyly that are characterized by an extra digit next to the little finger or the small toe. These conditions are called postaxial polydactyly type A (PAP-A) and type A/B (PAP-A/B). Another form of polydactyly, preaxial polydactyly type IV (PPD-IV), can also result from mutations in the GLI3 gene. People with this condition have extra digits next to the thumb or big toe (hallux) and fused skin between some fingers and toes (cutaneous syndactyly). PPD-IV also can include extra digits in other positions on the hands or feet. The pattern of polydactyly seen with PPD-IV is similar to that of Greig cephalopolysyndactyly syndrome, and some researchers suggest that PPD-IV may be a very mild form of that syndrome.
The GLI3 gene belongs to a family of genes that are involved in the normal shaping (patterning) of many tissues and organs during embryonic development. To carry out this role, proteins made by genes in the GLI family attach to specific regions of DNA and help control whether particular genes are turned on or off (gene expression). GLI proteins are called transcription factors on the basis of this action. Proteins in the GLI family function in the same molecular pathway as a protein called Sonic Hedgehog. This pathway is essential for early development. It plays a role in cell growth, cell specialization, and the patterning of structures such as the brain and limbs. Depending on signals from Sonic Hedgehog, the GLI3 protein can either turn on (activate) or turn off (repress) other genes. Researchers are working to identify the genes targeted by the GLI3 protein during development.
Conditions with Increased Gene Activity
Condition | Change (log2fold) | Comparison | Species | Experimental variables | Experiment name |
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Conditions with Decreased Gene Activity
Condition | Change (log2fold) | Comparison | Species | Experimental variables | Experiment name |
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Technical
The following transcription factors affect gene expression:
Tissue specificity:
Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.
Gene Pathways:
Molecular Function:
- Beta-Catenin Binding
- Chromatin Binding
- Histone Acetyltransferase Binding
- Histone Deacetylase Binding
- Metal Ion Binding
- Rna Polymerase Ii Core Promoter Proximal Region Sequence-Specific Dna Binding
- Rna Polymerase Ii Regulatory Region Sequence-Specific Dna Binding
- Transcriptional Activator Activity, Rna Polymerase Ii Core Promoter Proximal Region Sequence-Specific Binding
- Transcription Factor Activity, Sequence-Specific Dna Binding
Biological Processes:
- Anterior/Posterior Pattern Specification
- Anterior Semicircular Canal Development
- Artery Development
- Axon Guidance
- Branching Involved In Ureteric Bud Morphogenesis
- Camera-Type Eye Morphogenesis
- Cell Differentiation Involved In Kidney Development
- Developmental Growth
- Embryonic Digestive Tract Development
- Embryonic Digestive Tract Morphogenesis
- Embryonic Digit Morphogenesis
- Embryonic Skeletal System Morphogenesis
- Forebrain Dorsal/Ventral Pattern Formation
- Forebrain Radial Glial Cell Differentiation
- Frontal Suture Morphogenesis
- Heart Development
- Hindgut Morphogenesis
- Hippocampus Development
- In Utero Embryonic Development
- Lambdoid Suture Morphogenesis
- Lateral Ganglionic Eminence Cell Proliferation
- Lateral Semicircular Canal Development
- Layer Formation In Cerebral Cortex
- Limb Morphogenesis
- Lung Development
- Mammary Gland Specification
- Melanocyte Differentiation
- Metanephros Development
- Negative Regulation Of Alpha-Beta T Cell Differentiation
- Negative Regulation Of Apoptotic Process
- Negative Regulation Of Canonical Wnt Signaling Pathway
- Negative Regulation Of Cell Proliferation
- Negative Regulation Of Neuron Differentiation
- Negative Regulation Of Smoothened Signaling Pathway
- Negative Regulation Of Transcription, Dna-Templated
- Negative Regulation Of Transcription From Rna Polymerase Ii Promoter
- Negative Thymic T Cell Selection
- Nose Morphogenesis
- Odontogenesis Of Dentin-Containing Tooth
- Oligodendrocyte Differentiation
- Optic Nerve Morphogenesis
- Palate Development
- Positive Regulation Of Alpha-Beta T Cell Differentiation
- Positive Regulation Of Chondrocyte Differentiation
- Positive Regulation Of Neuroblast Proliferation
- Positive Regulation Of Osteoblast Differentiation
- Positive Regulation Of Protein Import Into Nucleus
- Positive Regulation Of Transcription, Dna-Templated
- Positive Regulation Of Transcription From Rna Polymerase Ii Promoter
- Protein Processing
- Proximal/Distal Pattern Formation
- Regulation Of Bone Development
- Response To Estrogen
- Sagittal Suture Morphogenesis
- Smoothened Signaling Pathway
- Smoothened Signaling Pathway Involved In Dorsal/Ventral Neural Tube Patterning
- Smoothened Signaling Pathway Involved In Spinal Cord Motor Neuron Cell Fate Specification
- Smoothened Signaling Pathway Involved In Ventral Spinal Cord Interneuron Specification
- T Cell Differentiation In Thymus
- Thymocyte Apoptotic Process
- Tongue Development
- Wound Healing