The Function of CLP1
Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
Protein names
Recommended name:
Polyribonucleotide 5'-hydroxyl-kinase Clp1Alternative name(s):
Polyadenylation factor Clp1Polynucleotide kinase Clp1
Pre-mRNA cleavage complex II protein Clp1
Top Gene-Substance Interactions
Substances That Increase CLP1
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Substances That Decrease CLP1
Substances | Interaction | Organism | Category |
Conditions with Increased Gene Activity
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Conditions with Decreased Gene Activity
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Technical
The following transcription factors affect gene expression:
Gene Pathways:
Molecular Function:
- Atp Binding
- Atp-Dependent Polydeoxyribonucleotide 5'-Hydroxyl-Kinase Activity
- Atp-Dependent Polyribonucleotide 5'-Hydroxyl-Kinase Activity
- Polydeoxyribonucleotide Kinase Activity
Biological Processes:
- Cerebellar Cortex Development
- Mrna 3'-End Processing
- Mrna Cleavage
- Mrna Polyadenylation
- Mrna Splicing, Via Spliceosome
- Sirna Loading Onto Risc Involved In Rna Interference
- Targeting Of Mrna For Destruction Involved In Rna Interference
- Termination Of Rna Polymerase Ii Transcription
- Trna Splicing, Via Endonucleolytic Cleavage And Ligation